Research Information System
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, vol.49, no.3, pp.144-146, 1994 (SCI-Expanded)
The LP system in raw milk was activated by the addition of equimolar concentrations (0.25 mM) of hydrogen peroxide (H2O2) and thiocyanate (SCN) as KSCN and utilizing the inherent milk lactoperoxidase (LP). H2O2 and SCN- concentrations were determined during the experiments by using spectrophotometric methods and found to decrease to 1.5 mug/ml and 0.4 mug/ml respectively in 5 h. A relatively small amount of H2O2 was produced in the untreated milk which then was found to decrease. The activated sample, at the end of the 5 h period contained 10(9) cells/ml whereas the control sample contained 10(11) cells/ml. Lactic acid (LA) concentration inceased from 0.24 to 0.58% in the control after a slight delay of 1 h, whereas in the LP activated sample LA remained at a level 0.23 for 5 h. The activated LP system was found to delay the coagulation time and to strongly reduce the activity of starter cultures.