Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine


Boyukbayram A. E. , Kiralp S., Toppare L. , Yagci Y.

BIOELECTROCHEMISTRY, vol.69, no.2, pp.164-171, 2006 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 69 Issue: 2
  • Publication Date: 2006
  • Doi Number: 10.1016/j.bioelechem.2006.01.006
  • Title of Journal : BIOELECTROCHEMISTRY
  • Page Numbers: pp.164-171
  • Keywords: conducting polymer, copolymer, biosensor, polyphenol oxidase, immobilization, electrochemistry, determination of phenolic compounds, red wine, ENZYME ELECTRODES, GRAFT-COPOLYMERS, POLYPYRROLE, POLYTETRAHYDROFURAN, TYROSINASE, COMPOSITE, THIOPHENE

Abstract

Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) and pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations were based on Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrical conductivities were measured by the four-probe technique.