Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyolojik Bilimler Bölümü, Türkiye
Tezin Onay Tarihi: 2016
Öğrenci: KÜBRA TOSUN
Danışman: KADRİ FATİH İZGÜ
Özet:Dermatopytosis is one of the most seen superficial fungal infections in humans resulting mild to severe inflammations. Antifungal drugs are not effective in the treatment of dermatophytosis due to serious adverse effects and resistance developed by fungal pathogens. Thus, the development of safe, naturally derived and potent antifungal/antimycotic agents which do not result in resistance and elicit little or no side effects to mammalian cells with high selectivity to the fungal pathogens is a continuing need. In this study, liposomal formulation of Panomycocin which is a novel and naturally derived killer protein produced by Pichia anomala was developed for treatment of human dermatophytosis. This study involves the preparation of Panomycocin loaded liposomes and their characterizations including in vitro biological activity test on tissue models against human dermatophytes. Panomycocin was encapsulated by stratum corneum lipid liposomes having lipid composition identical to that of the stratum corneum of mammalian cells where dermatophytes adhere to. The encapsulation and loading efficiencies of Panomycocin incorporated liposomes were calculated as 73 % and 76.8 %, respectively. TEM images revealed that liposome nanoparticles were in nano size with roughly spherical shape. Zeta potential and XRD analyses showed that liposomes were stable in collodial system and amorphous, respectively. Presence of covalent interactions between Panomycocin and liposomes were not observed by FTIR. DLS proved that liposomes were monodisperse in suspension without any aggregation. In vitro release tests determined that 1.71 % of Panomycocin was released from liposomes within 12 hours. The biological activity test on Reconstructed Human Epidermis tissue models against human dermatophytes indicated that developed formulation had an inhibitory effect on tested dermatophytes at similar MFC values which was previously published for pure Panomycocin. Liposomal formulation of Panomycocin prepared with stratum corneum lipids led up to further preclinical and clinical studies for the treatment of dermatophytosis.