Enzymatic hydrolysis of fruit peels and other lignocellulosic biomass as a source of sugar for fermentation

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, Türkiye

Tezin Onay Tarihi: 2015

Öğrenci: PELİN POÇAN

Eş Danışman: HALİL MECİT ÖZTOP, HALUK HAMAMCI

Özet:

In this study, enzymatic hydrolysis of corn cobs, orange and pomegranate peels were evaluated. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined and it was found that glucose and reducing sugar yield (%) for the pretreated corn cobs increased from 7% to 21.5% and 14% to 33.6% respectively. Effect of cellobiase loading on hydrolysis efficiency was also investigated. It was observed that when cellobiase was added to hydrolysate in addition to cellulase, glucose and total reducing sugar yield increased from 11% to 20% and 21% to 33.5% respectively. On the other hand, although cellulase amount was increased successively while cellobiase was kept at constant load, glucose and total reducing sugar yield did not change and was constant at around 20% and 35% respectively. Due to their either low lignin content or high sugar content, alkaline pretreatment was not preferred for the pomegranate and orange peels. For the hydrolysis of these substrates, in addition to cellulolytic enzymes pectic enzymes were also used. It was found that when pectinase was used in addition to cellulase, conversion to glucose and total reducing sugar (%) increased significantly.