Investigation of the therapeutic effect of sodium butyrate in Caco2 colon cancer cell line by using ATR-FTIR spectroscopy

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Fizik Bölümü, Türkiye

Tezin Onay Tarihi: 2018

Öğrenci: BUKET ÇELİK

Danışman: ALPAN BEK

Özet:

Sodium butyrate (NaBt), as one of the HDACi, has been demonstrated that it induces apoptosis, cell cycle arrest, the inhibition of angiogenesis, metastasis and gene expression changes. To date, there are several studies perfomed to investigate its therapeutic effect; however, theexact mechanism at molecular level is not clear yet. Therefore, the current thesis was aimed to clarify the action/theurapeutic potential mechanisms of sodium butyrate in Caco2 colon cancer cell line at molecular level using ATR-FTIR spectroscopy, quantitative spectral and chemometric analyses. 3mM, 6mM and 9mM doses of sodium butyrate were applied to Caco2 cell line line for 12h, 24h and 48 h. According to the results, NaBt treatment caused an increase in the unsaturated lipids, impying elevated lipid peroxiation. Moreover increased saturated lipids, membrane fluidity/dynamics and esters and triacylglycerols were obtained in NaBt-treated groups with respect to the control ones. However, NaBt induced a degradation of proteins and nucleic acids. HCA and PCA results indicated that in all studied doses, there was a clear distinction between studied groups in a time dependent manner. The results of the current study implied that sodium butyrate may have therapeutic potential by structural and functional remodelling of cancer cells.