Local delivery of bioactive agents for bone tissue engineering

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Mühendislik Fakültesi, Mühendislik Bilimleri Bölümü, Türkiye

Tezin Onay Tarihi: 2017

Öğrenci: HANI ALISSA ALAM

Danışman: DİLEK KESKİN

Özet:

Many cases of bone defects require bone grafts addition into the body. Bone tissue substitutes have more advantages over other bone grafts because of low infection risk and abundance of materials. Local delivery of drugs or bioactive agents can help in these diseases’ treatments and bone growth overall while avoiding side effects or drug interference. To accelerate local bone regrowth, a research about developing a controlled local release system of bioactive agents to bone cells was conducted in this study using bone tissue substitute as a template for the controlled local release system. Wet (Monoaxial) electrospinning can produce 3D scaffolds that can either deliver a hydrophilic or a hydrophobic bioactive agent. However, to fabricate a 3D scaffold that can hold and maintain both for local delivery, and has the desired bone graft scaffold characteristics, a coaxial wet electrospinning system was used for the first time in bone tissue engineering. After optimization studies on scaffolds made from different combinations of polymer solutions and various electrospinning systems, four groups were chosen based on some micro and macro properties of their respective scaffolds. Additional studies, including release profiles tests and In situ and In vitro characterizations, were carried out to evaluate their bone tissue engineering related properties and see their potency as local drug delivery system using vitamin K2 and calcitonin as a hydrophobic and a hydrophilic bioactive agent respectively. One of these scaffolds demonstrate better characteristics, but further studies to enhance its mechanical properties and optimize the bioactive agents’ dosages are needed.