Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Türkiye
Tezin Onay Tarihi: 2023
Tezin Dili: İngilizce
Öğrenci: HATİCE TUĞBA SELVİ
Danışman: Serhan Türkyılmaz
Özet:
The discovery and widespread clinical use of antibiotics have been one of the most important developments in medicine. As a result, morbidity and mortality associated with infectious diseases caused by pathogenic bacteria have been reduced dramatically. The widespread and frequently unnecessary use of antibiotics has resulted in the frequent and continuous emergence of bacterial strains resistant to antibiotics in clinical use. As a result, whole classes of antibiotics are becoming obsolete ,and now pathogenic bacterial strains exist that do not respond to known antibiotics. Antibiotic-resistant bacteria are one of the most important risks to public health.
There are several approaches that could be taken to tackle this problem. One approach is to enhance the selectivity of antibiotic agents by conjugating them with bacterial targeting groups to widen their therapeutic window. Zinc(II) bisdipicolylamine (Zn2(BDPA)) complexes, which are known to bind to bacterial membranes and walls, can be used for this purpose. Another approach is antibacterial photodynamic therapy (PDT). In PDT a photosensitizer (e.g., porphyrins) is used to absorb light and generate singlet oxygen (1O2) which is highly toxic towards bacterial cells, although this toxicity is not cell-specific. The probability of emergence of widespread bacterial resistance towards 1O2 is low. Yet another potential approach is to remove bacterial from liquids, particularly blood, and thus reduce the infective potential.
The aims of the studies summarized in this thesis are three-fold: The first objective is to evaluate the PDT efficacy of a number of Zn2(BDPA) complex bearing porphyrin derivatives against a number bacterial strains. The second objective is to develop Zn2(BDPA) complex bearing liposomes and to explore their antibiotic potential. The third is to develop Zn2(BDPA) complex bearing glass microspheres for sequestration of bacterial cells from liquids, blood in particular.