Effect of boron and iron stress on phytosiderophore production in barley

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2015

Öğrenci: YETKİN ÇAKA İNCE

Danışman: AYŞE MERAL YÜCEL

Özet:

Boron and iron are essential nutrients for growth and development of higher plants. Deficiency and toxicity of them cause significant changes in physiological, cellular and molecular make-up of plants. Iron uptake in barley is mainly achieved by production and release of phytosiderophores to the environment. On the other hand, production of phytosiderophores are affected by concentrations of boron and iron. Four enzymes which function in phytosiderophore hydroxymugineic acid (HMA) production namely NAS, NAAT, IDS2, and IDS3 showed differential expression profiles when plants were treated with different concentrations of iron and boron. In this study, we investigated the potential link between iron, boron, and phytosiderophore production in barley. For this purpose the barley seedlings were treated with deficiency or toxicity of boron and iron as well as combinations of these and expression levels of aforementioned genes were examined.In addition to gene expression analysis, physiological parameters, total boron, iron, and zinc content and photosynthetic performance of seedlings were considered.