Wet spun PCL scaffolds for tissue engineering

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Rektörlük, Rektörlük, Türkiye

Tezin Onay Tarihi: 2017

Tezin Dili: İngilizce

Öğrenci: ELBAY MALIKMAMMADOV

Asıl Danışman (Eş Danışmanlı Tezler İçin): Nesrin Hasırcı

Eş Danışman: Tuğba Endoğan Tanır

Özet:

Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investigated against Escherichia coli (E. coli), chosen as a model gram-negative bacterium. β-TCP and gelatin were added to enhance mechanical properties while directing the scaffold towards osteogenic infrastructure; and to increase hydrophilicity by activating cell attachment via protein molecules, respectively. Modifications with β-TCP and gelatin enhanced compression modulus about 70%, and attachment of Saos-2 cells for 60%, respectively. Presence and release of CS demonstrated effective antimicrobial activity against E. coli. Bioactive scaffolds prepared in this study can be good candidates for bone tissue engineering applications.