Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyolojik Bilimler Bölümü, Türkiye
Tezin Onay Tarihi: 2014
Tezin Dili: İngilizce
Öğrenci: Müşerref Şeyma Ceyhan
Danışman: SREEPARNA BANERJEE
Özet:Cancer is a multi-factorial disease that may arise as a consequence of different factors such as age, diet, and environmental factors as well as genetic makeup. Moreover, cancerous cells can develop certain strategies to evade immune system and develop resistance against chemotherapy drugs. Therefore, multi-targeted therapeutics have gained importance for both prevention of cancer and better prognosis. Extracts from Salix aegyptiaca includes several active phytochemicals; phenolic compounds and flavonoids and therefore have the potential for use as herbal remedies. The aim of this study was to investigate antioxidant properties and anticarcinogenic effects of ethanolic extract from bark (EEB) of Salix aegyptiaca and its fractions in colon cancer. EEB was fractionated into different fractions by solvent solvent partitioning. The antioxidant property of EEB and its fractions were investigated by 1, 1-diphenyl -2-picryl hydrazyl (DPPH) free radical scavenging activity, total phenolic and flavonoid content assays. Effect of EEB and its active fractions on cancer cell proliferation were evaluated by MTT and BrdU incorporation assays. The ethyl acetate fraction showed the highest free radical scavenging activity (11 ± 1 μg/ml) and total phenolic content (29.6 ± 2 mg GAE/100 g dried weight). EEB had the highest total flavonoid content (47.5 ± 2 mg of EGCG equivalent/100 g dried weight) compared to its fractions. EEB, ethyl acetate and aqueous fractions decreased proliferation of cancer cells. Catechin, catechol and salicin were detected in high quantity in the ethyl acetate and aqueous fractions by tandem mass spectrometry (MS). Treatment of cancer cells with a combination of catechin and catechol had higher efficacy in reduction of proliferation than the individual pure compounds. EEB led to significant decrease in phosphorylation of ERK1/2, JNK1/2 and p38, but its active fractions were only functional in reducing the phosphorylation of ERK1/2. To conclude, our data suggest that EEB and its active fractions could be thought of as a potent antioxidant and anticarcinogenic agent that may be used in complementary and alternative medicine.