Investigation of structural and functional properties of OmpG mutants by FTIR spectroscopy

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Fizik Bölümü, Türkiye

Tezin Onay Tarihi: 2017

Öğrenci: İREM YILMAZ

Danışman: ALPAN BEK

Özet:

The subject of the current study is a bacterial protein OmpG from Escherichia coli. OmpG-16S and OmpG-16SL are the mutants of OmpG that are genetically engineered for potential biotechnological applications. In this study, structural and functional similarities and/or differences of these mutants with respect to the wild type OmpG were revealed using IR spectroscopy. Also, a new spectrometer attachment enabling in situ 1H/2H exchange was developed. For the collection of mass amount of spectral data from each experiment, an automated data collection computer code was developed as well.