Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Fen Bilimleri Enstitüsü, Türkiye
Tezin Onay Tarihi: 2012
Öğrenci: TUĞBA KESKİN
Danışman: UFUK GÜNDÜZ
Özet:Conventional cancer chemotherapies cannot differentiate between healthy and cancer cells, and lead to severe side effects and systemic toxicity. In the last decades, different kinds of controlled drug delivery systems have been developed to overcome these shortcomings of chemotherapeutics. Magnetic nanoparticles (MNP) are potentially important in cancer treatment since they can be targeted to tumor site by an externally applied magnetic field. In this study, it is aimed to synthesize folic acid conjugated; polyethylene glycol (PEG) coated magnetic nanoparticles with appropriate size, surface chemistry, magnetization and biocompatibility to be used in biomedical applications. First MNP were synthesized, then covered with oleic and PEG; and finally conjugated with folic acid. A detailed characterization of synthesized nanoparticles was done by TEM, XRD, FTIR, VSM and XTT analyses. MNP synthesized by the rapid addition of ammonium hydroxide exhibited more spherical nanoparticles with a narrower size distribution. Agglomeration tendency of naked nanoparticles was prevented by oleic acid addition during the synthesis. Both naked and surface treated MNP have been found to exhibit superparamagnetic behavior both at room temperature (23˚C) and body temperature (37˚). Cytotoxic effects of naked MNP, oleic acid coated MNP, PEG coated MNP and folic acid functionalized MNP were investigated on MCF-7/S breast cancer cell lines. They did not exhibit severe toxicity in the concentration range of 0 – 250 µg/ml. Cell proliferation profiles of drug resistant cell lines MCF-7/Dox, MCF-7/Pac and MCF-7/Zol were examined for folic acid conjugated MNP and a serious cytotoxicity was not observed. Endocytosis of MNP was illustrated by light microscopy images. The synthesized nanoparticles have been found to be suitable in terms of size, shape, magnetic and cytotoxic properties for drug targeting or other biomedical applications.