Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Türkiye
Tezin Onay Tarihi: 2009
Tezin Dili: İngilizce
Öğrenci: Ömer Faruk Gerdan
Danışman: NURSEN ÇORUH
Özet:Fresh produces, fruit juices and herbal teas used in our regular diet may have importance in the protective treatment of some infectious diseases. In this study, dietary produces were investigated for their antioxidant activities and antimicrobial activities against group A ß-haemolytic streptoccoci. Streptococcus pyogenes, a member of the group A ß-haemolytic streptococci, is a very dangerous pathogen, which may cause diseases such as tonsillopharyngitis, meningitis, rheumatic arthritis. Fruits and vegetables; onion, radish, carrot, plum, fruit juices; orange, peach, pomegranate, grape and teas; sage, anise, rosehip, chamomile were chosen as samples of regular daily diets. Dry extracts were obtained either by lyophilizing or fractionating in ethyl acetate. Antioxidant activities of extracts were examined by total phenolic content determination, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) methods. Antimicrobial activities of extracts were studied by disk diffusion test, minimum inhibitory and bactericidal concentration methods. Sage, plum, onion and radish displayed high radical scavenging activity with EC50 values of 0.043, 0.049, 0.148 and 0.414 mg/mL, respectively. Plum, sage, onion and radish were found high in total phenolic contents with μg gallic acid equivalent of 50.506, 48.299, 44.427 and 13.135 in mg extract, respectively. High antimicrobial activities were obtained by onion, radish, anise, carrot and peach extracts as tested by disk diffusion method with respective 20, 16, 16, 14 and 14 millimeters clear growth inhibition zones. Carrot, onion and radish extracts were found as effective bacteriostatic and bactericidal agents with minimum inhibitory and bactericidal respective concentrations of 0.008, 0.125, 0.250 mg/mL and 0.06, 0.5, 1 mg/mL.