Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

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Kara N., Ayoub N., Ilgu H., Fotiadis D., İLGÜ M.

Molecules, cilt.28, sa.9, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 28 Sayı: 9
  • Basım Tarihi: 2023
  • Doi Numarası: 10.3390/molecules28093728
  • Dergi Adı: Molecules
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: aptamers, aptasensor, biosensor, diagnostics, membrane proteins, SELEX, SURFACE-PLASMON RESONANCE, GROWTH-FACTOR RECEPTOR, IN-VITRO SELECTION, POST-SELEX OPTIMIZATION, CIRCULATING TUMOR-CELLS, BREAST-CANCER CELLS, DNA APTAMERS, RNA APTAMER, LABEL-FREE, HIGH-AFFINITY
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These “aptasensors” can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.