Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase


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Douglas T. E. L. , Messersmith P. B. , Chasan S., Mikos A. G. , de Mulder E. L. W. , Dickson G., ...Daha Fazla

MACROMOLECULAR BIOSCIENCE, cilt.12, ss.1077-1089, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 12 Konu: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/mabi.201100501
  • Dergi Adı: MACROMOLECULAR BIOSCIENCE
  • Sayfa Sayıları: ss.1077-1089

Özet

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.