Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

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Douglas T. E. L., Messersmith P. B., Chasan S., Mikos A. G., de Mulder E. L. W., Dickson G., ...Daha Fazla

MACROMOLECULAR BIOSCIENCE, cilt.12, sa.8, ss.1077-1089, 2012 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/mabi.201100501
  • Dergi Adı: MACROMOLECULAR BIOSCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1077-1089
  • Anahtar Kelimeler: biomaterials, biomineralization, composites, enzymes, hydrogels, GLYCOL) FUMARATE) HYDROGELS, COLLAGEN TYPE-I, CALCIUM-PHOSPHATE, SUBSTRATE STIFFNESS, HYDROXYAPATITE, DIFFERENTIATION, COMPOSITES, CELLS, RAT, METHACRYLATE
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.