Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase


Creative Commons License

Douglas T. E. L. , Messersmith P. B. , Chasan S., Mikos A. G. , de Mulder E. L. W. , Dickson G., ...More

MACROMOLECULAR BIOSCIENCE, vol.12, no.8, pp.1077-1089, 2012 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 8
  • Publication Date: 2012
  • Doi Number: 10.1002/mabi.201100501
  • Journal Name: MACROMOLECULAR BIOSCIENCE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1077-1089
  • Keywords: biomaterials, biomineralization, composites, enzymes, hydrogels, GLYCOL) FUMARATE) HYDROGELS, COLLAGEN TYPE-I, CALCIUM-PHOSPHATE, SUBSTRATE STIFFNESS, HYDROXYAPATITE, DIFFERENTIATION, COMPOSITES, CELLS, RAT, METHACRYLATE

Abstract

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.