Use of a thiophene-based conducting polymer in microbial biosensing

ODACI DEMİRKOL D., Kayahan S. K., TİMUR S., Toppare L.

ELECTROCHIMICA ACTA, cilt.53, sa.12, ss.4104-4108, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 12
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.electacta.2007.12.065
  • Dergi Adı: ELECTROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4104-4108
  • Anahtar Kelimeler: conducting polymer, electrochemical polymerization, microbial electrode, pseudomonas fluorescens, electrochemical biosensor, PSEUDOMONAS-PUTIDA, IMMOBILIZATION, TYROSINASE, POLY(3-ALKYLTHIOPHENES), POLYANILINE, COPOLYMERS, FILMS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Immobilization of whole viable Pseudomonas fluorescens cells was achieved on a graphite electrode modified with a thiophene-based conducting polymer. Microbial electrodes were constructed by the entrapment of bacterial cells on conducting copolymer matrix using a dialysis membrane. The biosensor was characterized using glucose as the substrate. As well as analytical characterization, effects of electropolymerization time, pH and temperature on the sensor response were examined. Finally, operational stability was also tested. (c) 2008 Elsevier Ltd. All rights reserved.