Proceedings of the 2nd Conference on Advances in Biochemical Engineering, Brighton, İngiltere, 4 - 06 Temmuz 1994, ss.151-153
Kluyveromyces spp were found to be more efficient than the closely-related yeast Saccharomyces cerevisiae in their potential to accumulate heavy metals and K. marxianus was the most promising organism. The subcellular distribution of the accumulated metals in S. cerevisiae and K. marxianus was next investigated to reveal the localization of the metals in these organisms. S. cerevisiae contained relatively more metal in its cytosol. 92.3% of the initial silver in medium has been accumulated by K. marxianus, but only 5% of this was associated with soluble cytoplasm. By contrast, 10% of the cell-bound silver was in cytosol in S. cerevisiae while silver accumulation by this organism was only 39%. With also copper and cadmium, binding to the insoluble material rather than to metallothionein accounted for metal accumulation in both organisms.