JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.93, no.11, pp.3326-3335, 2018 (SCI-Expanded)
Article / Article
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Science Citation Index Expanded (SCI-EXPANDED), Scopus
hybrid fed-batch, bioreactor operation, high-cell-density fermentation, substrate uptake, fed-batch, Pichia pastoris, GAP promoter, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE PROMOTER, METHANOL FEEDING STRATEGY, PICHIA-PASTORIS, GAP PROMOTER, EXPRESSION, SECRETION, MOLASSES, SYSTEMS
Middle East Technical University Affiliated:
BACKGROUNDThe hybrid fed-batch bioreactor (HFBB) operation is a novel design method for high-cell-density fermentations. It employs fed-batch and batch operations, starting with fed-batch that in turn shifts to the next, and proceeds in successive cycles to enhance production and productivity by controlling the substrate uptake rate.