Synthesis of DNA-encapsulated silica elaborated by sol-gel routes


Kapusuz D., DURUCAN C.

JOURNAL OF MATERIALS RESEARCH, cilt.28, ss.175-184, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 28 Konu: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1557/jmr.2012.309
  • Dergi Adı: JOURNAL OF MATERIALS RESEARCH
  • Sayfa Sayıları: ss.175-184

Özet

The highly specific functions of DNA can be used for designing novel functional materials. However, aqueous solubility and biochemical instability of DNA impede its direct utilization as a functional component. Herein, preparation of a hybrid material encapsulating the DNA molecules (double-stranded salmon sperm, 50-5000 base pairs) in robust host-sol-gel-derived silica-has been described. The encapsulation was carried out in two steps: hydrolysis of an acidic tetraethylorthosilicate [Si(OC2H5)(4)] sol and was followed by condensation near physiological pH upon addition of alkaline DNA-containing solutions. The gelation behavior and structural properties of the DNA-silica hybrids were investigated by Si-29 nuclear magnetic resonance and by nitrogen adsorption. The selective adsorption of a DNA-interactive reagent molecule (ethidium bromide) in their diluted aqueous solutions on DNA-silica hybrids confirmed that the DNA molecules remained entrapped within the silica host without any deterioration. A DNA encapsulation mechanism correlating the silica microstructure and DNA holding efficiency has been proposed.