Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)


Baloglu M. C., Oz M. T., ÖKTEM H. A., YÜCEL A. M.

PLANT MOLECULAR BIOLOGY REPORTER, cilt.30, sa.5, ss.1246-1252, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 5
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s11105-012-0445-3
  • Dergi Adı: PLANT MOLECULAR BIOLOGY REPORTER
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1246-1252
  • Anahtar Kelimeler: NAC-type transcription factors, Wheat, Gene expression, RT-qPCR, MOLECULAR-CLONING, SALT TOLERANCE, ARABIDOPSIS, DROUGHT, SENESCENCE, RESPONSES, PROTEIN
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses.