Design of a microbial sensor using conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine

Tuncagil S., ODACI DEMİRKOL D., Yidiz E., TİMUR S., Toppare L.

SENSORS AND ACTUATORS B-CHEMICAL, vol.137, no.1, pp.42-47, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 137 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1016/j.snb.2008.10.067
  • Journal Name: SENSORS AND ACTUATORS B-CHEMICAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.42-47
  • Keywords: Conducting polymers, Microbial biosensors, Electrochemical polymerization, Electrochemical biosensors, GLUCONOBACTER-OXYDANS, BIOSENSOR, ETHANOL, FERMENTATION, GLYCEROL, GLUCOSE, CELLS
  • Middle East Technical University Affiliated: Yes

Abstract

A microbial biosensor based on Gluconobacter oxydans cells immobilized on the conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) coated onto the Surface of graphite electrode was constructed. The proposed biosensor was characterized using glucose as the Substrate. The linear relation was observed in the range of 0.1-2.5 mM and defined by the equation y = 0.415x + 0.377 (R-2 = 0.986). Analytical characterization, effects of electropolymerization time, pH, cell amount and the presence of gold nanoparticles (GNP) on the polymer surface together with the biological material were examined as well. Finally, the system was used for ethanol and glucose detection in real samples. (c) 2008 Elsevier B.V. All rights reserved.