© The Author(s) 2022.The first purpose of this study was, as the first time in the literature, to investigate usability of polylactide (PLA) biopolymer as the matrix of high-performance composite laminated structures having woven forms of glass and carbon fibers. For this purpose, 2-14 layers of 2 × 2 twill Carbon Fiber (CF) and 1 × 1 plain weave Glass Fiber (GF) forms were stacked by PLA powders followed by consolidation of these layers by compression molding technique. After conducting various tests and analysis it was observed that significant improvements in the mechanical and thermal properties could be obtained by laminating PLA with woven CF and GF layers. For instance, mechanical properties obtained for PLA/CF laminates with 14 layers were flexural strength of 641 MPa, flexural modulus of 34 GPa and interlaminar shear strength of 38 MPa. Another purpose of this study was to compare mechanical performance of the PLA matrix laminates with Epoxy matrix laminates having exactly the same type and number of GF and CF layers. It was revealed that mechanical properties of the PLA/CF laminates were approaching to the values of the Epoxy/CF laminates (e.g. flexural strength of 607 and 673 MPa, respectively); while due to poor adhesion between the PLA matrix and Glass Fiber surfaces, the properties were lower in the PLA/GF laminates (e.g. flexural strength of 302 and 553 MPa, respectively). Therefore, it could be generally concluded that, in terms of mechanical performance; traditional thermoset Epoxy matrix could be replaced with the renewable biopolymer PLA matrix in the Woven Carbon Fiber laminates. For the Woven Glass Fiber laminates, a proper sizing treatment with a PLA compatible silane coupling agent would be necessary.