PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES


EROGLU I. , AYDEMIR A., TURKER L., YUCEL A.

JOURNAL OF MEMBRANE SCIENCE, vol.86, pp.171-179, 1994 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 86
  • Publication Date: 1994
  • Doi Number: 10.1016/0376-7388(93)e0138-a
  • Title of Journal : JOURNAL OF MEMBRANE SCIENCE
  • Page Numbers: pp.171-179

Abstract

A two-chambered cell has been designed and constructed for the measurement of photoresponses of bacteriorhodopsin immobilized in polyacrylamide gel (FAG) membranes. The photoresponse of bacteriorhodopsin was determined by measuring the pH variation in each chamber independently, for long periods of illumination. The aim was to verify the light driven proton transfer of bacteriorhodopsin immobilized in FAG membranes. However, the same pH variation was observed in the two chambers irrespective of whether oriented or nonoriented bacteriorhodopsin immobilized in FAG membranes were employed.