PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES

EROGLU I., AYDEMIR A., TURKER L., YUCEL A.

JOURNAL OF MEMBRANE SCIENCE, vol.86, pp.171-179, 1994 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 86
  • Publication Date: 1994
  • Doi Number: 10.1016/0376-7388(93)e0138-a
  • Journal Name: JOURNAL OF MEMBRANE SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.171-179
  • Middle East Technical University Affiliated: No

Abstract

A two-chambered cell has been designed and constructed for the measurement of photoresponses of bacteriorhodopsin immobilized in polyacrylamide gel (FAG) membranes. The photoresponse of bacteriorhodopsin was determined by measuring the pH variation in each chamber independently, for long periods of illumination. The aim was to verify the light driven proton transfer of bacteriorhodopsin immobilized in FAG membranes. However, the same pH variation was observed in the two chambers irrespective of whether oriented or nonoriented bacteriorhodopsin immobilized in FAG membranes were employed.