Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole

Sahmetlioglu E., Yuruk H., Toppare L., Cianga I., Yagci Y.

REACTIVE & FUNCTIONAL POLYMERS, cilt.66, sa.3, ss.365-371, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: 3
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.reactfunctpolym.2005.08.009
  • Dergi Adı: REACTIVE & FUNCTIONAL POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.365-371
  • Anahtar Kelimeler: immobilization, invertase, glucose oxidase, poly(vinyl alcohol), electropolymerization
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was examined. The optimum temperature, operational and storage stabilities of immobilized enzymes were determined. PVATh/PPy copolymer was found to exhibit significantly enhanced properties compared to pristine polypyrrole. (c) 2005 Elsevier B.V. All rights reserved.