Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers

Gursel A., Alkan S., Toppare L., Yagci Y.

REACTIVE & FUNCTIONAL POLYMERS, vol.57, no.1, pp.57-65, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 1
  • Publication Date: 2003
  • Doi Number: 10.1016/j.reactfunctpolym.2003.07.004
  • Journal Name: REACTIVE & FUNCTIONAL POLYMERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.57-65
  • Keywords: immobilization, invertase, glucose oxidase, polysiloxane, electropolymerization, POLYPYRROLE FILMS, POLYMER MATRICES, GRAFT-COPOLYMERS, ENZYMES, ENTRAPMENT
  • Middle East Technical University Affiliated: Yes

Abstract

In this study, immobilizations of enzymes, invertase and glucose oxidase, were achieved in conducting copolymers of N-pyrrolyl terminated polydimethylsiloxane/polypyrrole (PDMS/PPy) matrices via electrochemical polymerization. The kinetic parameters, v(max) (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on enzyme activity and film morphologies was examined. The optimum temperatures and operational stabilities of immobilized enzymes were determined. PDMS/PPy copolymer matrix was found to exhibit significantly enhanced properties compare to pristine polypyrrole in terms of relative enzyme activities, kinetic parameters and operational stabilities. (C) 2003 Published by Elsevier B.V.