Immobilization of glucose oxidase onto gelatin for biosensor construction

Emregul E., Sungur S., Akbulut U.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, cilt.16, sa.4, ss.505-519, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Konu: 4
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1163/1568562053700138
  • Sayfa Sayıları: ss.505-519


The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized using chemical and electrochemical methods. Temperature, pH, cross-linking agent concentration, enzyme concentration, kinetic properties, reusability and the effect of electro-active compounds were among the parameters studied. The response time of the glucose oxidase biosensor is 90 s, the detection limit is below 1 mmol/dm(3) and the sensor can be used 20 times within a 2-month period without losing its stability.