Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices

Alkan S., Toppare L., Bakir U., Yagci Y.

SYNTHETIC METALS, cilt.123, sa.1, ss.95-99, 2001 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123 Sayı: 1
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1016/s0379-6779(00)00580-4
  • Dergi Adı: SYNTHETIC METALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.95-99
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Urease was immobilized in conducting polypyrrole and block copolymers of thiophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml supporting electrolyte. The optimum immobilization conditions, electrolysis time, electrolysis medium, enzyme concentration during electrolysis, type of supporting electrolyte were determined. The kinetic behaviors of free and immobilized urease were determined. (C) 2001 Elsevier Science B.V. All rights reserved.