KINETIC-PROPERTIES OF PURIFIED SHEEP LUNG MICROSOMAL NADH-CYTOCHROME B5 REDUCTASE


GURAY T. , ARINC E.

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, cilt.23, ss.1315-1320, 1991 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 23 Konu: 11
  • Basım Tarihi: 1991
  • Doi Numarası: 10.1016/0020-711x(91)90233-d
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOCHEMISTRY
  • Sayfa Sayıları: ss.1315-1320

Özet

1. Lung NADH-cytochrome b5 reductase was saturated with its artificial substrate, potassium ferricyanide at approximately 0.1 mM ferricyanide concentration, and the activity of the lung enzyme was inhibited by the higher concentrations of potassium ferricyanide. Ferricyanide at 0.5 and 1.0 mM inhibited the activity of the enzyme by about 20 and 61% respectively. The apparent K(m) value was calculated as 13.7-mu-M potassium ferricyanide and 4.3-mu-M NADH.