Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening

Gomez-Rios G. A., Tascon M., Reyes-Garces N., Boyaci E., Poole J., Pawliszyn J.

SCIENTIFIC REPORTS, vol.7, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7
  • Publication Date: 2017
  • Doi Number: 10.1038/s41598-017-16494-z
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Middle East Technical University Affiliated: Yes


This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 mu L of biofluid, with limits of quantitation in the low/sub ng.mL(-1) level attained within 7 minutes.