Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics

Pyeshkova V. M., Dudchenko O. Y., Soldatkin O. O., Kasap B. O., Lagarde F., AKATA KURÇ B., ...More

NANOSCALE RESEARCH LETTERS, vol.10, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10
  • Publication Date: 2015
  • Doi Number: 10.1186/s11671-015-0853-z
  • Journal Name: NANOSCALE RESEARCH LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Sucrose biosensor, Silicalite, Conductometric transducer, ZEOLITE
  • Middle East Technical University Affiliated: Yes

Abstract

The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/ silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.