Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose


Ifrij I., Ogel Z.

BIOTECHNOLOGY LETTERS, cilt.24, sa.13, ss.1107-1110, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 13
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1023/a:1016026904687
  • Dergi Adı: BIOTECHNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1107-1110
  • Anahtar Kelimeler: endoglucanase, protease, Scytalidium thermophilum, thermophilic fungus, ENDOGLUCANASE PRODUCTION, PURIFICATION, HUMICOLA
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors.