Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose
BIOTECHNOLOGY LETTERS, cilt.24, sa.13, ss.1107-1110, 2002 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 24 Sayı: 13
- Basım Tarihi: 2002
- Doi Numarası: 10.1023/a:1016026904687
- Dergi Adı: BIOTECHNOLOGY LETTERS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1107-1110
- Anahtar Kelimeler: endoglucanase, protease, Scytalidium thermophilum, thermophilic fungus, ENDOGLUCANASE PRODUCTION, PURIFICATION, HUMICOLA
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors.