Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose
BIOTECHNOLOGY LETTERS, vol.24, no.13, pp.1107-1110, 2002 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 24 Issue: 13
- Publication Date: 2002
- Doi Number: 10.1023/a:1016026904687
- Journal Name: BIOTECHNOLOGY LETTERS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.1107-1110
- Keywords: endoglucanase, protease, Scytalidium thermophilum, thermophilic fungus, ENDOGLUCANASE PRODUCTION, PURIFICATION, HUMICOLA
- Middle East Technical University Affiliated: Yes
Abstract
Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors.