Real-Time PCR Investigation of the Dynamic Expression of Three "RNA Processing and Modification" Genes of Phanerochaete chrysosporium Exposed to Lead

Tekın E., İçgen B. , Özcengiz G.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol.90, pp.27-33, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 90
  • Publication Date: 2013
  • Doi Number: 10.1007/s00128-012-0886-2
  • Page Numbers: pp.27-33


The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 mu M lead, respectively. 50 and 100 mu M lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 mu M lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.