Thermal inactivation of native and crosslinked invertases

Kaplan O., Bakir U., Erarslan A.

BIOTECHNOLOGY TECHNIQUES, vol.11, no.9, pp.621-623, 1997 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 9
  • Publication Date: 1997
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.621-623
  • Middle East Technical University Affiliated: No


Formaldehyde and three homobifunctional bisimidoesters having different lengths, dimethyl suberimidate (DMS), dimethyl adipimidate (DMA), dimethyl-3,3'-dithiobispropionimidate (DTBP), were used to crosslink invertase. Optimal concentrations for crosslinking with DMS and formaldehyde were determined as 0.3% and 0.015%, respectively. Inactivation mechanisms of the native, DMS and formaldehyde cross-linked invertases were first order during prolonged heat treatment. The maximum stabilization factors with DMS and formaldehyde were 1.5 at 55 degrees C and 1.7 at 57.5 degrees C, respectively. V-max and K-m values decreased 5 to 10% by DMS and formaldehyde crosslinking. On the other hand, crosslinking with DMA and DTBP decreased stability against inactivation.