Genotyping of Anatolian doubled-haploid durum lines with SSR markers


Hakki E., Savaskan C., Akkaya M.

EUPHYTICA, vol.122, no.2, pp.257-262, 2001 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 122 Issue: 2
  • Publication Date: 2001
  • Doi Number: 10.1023/a:1012955516198
  • Title of Journal : EUPHYTICA
  • Page Numbers: pp.257-262
  • Keywords: doubled-haploid durum wheat, fingerprinting, genetic relationships, SSR (microsatellites)

Abstract

In this study, doubled haploid lines generated from the durum wheat varieties, selections from Middle Anatolian landraces, 'Cakmak-79', 'Berkmen-469' and 'Kunduru-1149' (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The average PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars 'Cakmak-79' and 'Kunduru-1149', were the two most distant populations with (delta mu)(2) = 1.42. 'Berkmen-469' x 'Cakmak-79' and 'Berkmen-469' x 'Kunduru-1149' yielded similar genetic distances, (delta mu)(2) of 0.84 and 0.85, respectively. In addition, the genetic relationship between the progenitors of the DH lines together with other durum wheat varieties was analyzed. A meaningful relationship was obtained based on available pedigree information on the cultivars.