PRODUCTION OF THE LEPIDOPTERA-SPECIFIC CRYSTAL PROTEIN BY A LOCAL ISOLATE OF BACILLUS-THURINGIENSIS

AFKHAMI P., OZCENGIZ G., ALAEDDINOGLU N.

BIOTECHNOLOGY LETTERS, vol.15, no.12, pp.1253-1258, 1993 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 12
  • Publication Date: 1993
  • Doi Number: 10.1007/bf00130307
  • Journal Name: BIOTECHNOLOGY LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1253-1258
  • Middle East Technical University Affiliated: Yes

Abstract

A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25-degrees-C and the lowest at 42-degrees-C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.