The development of green and safe processes for the synthesis of nanomaterials is one of the main aspects of nanotechnology. In this study, a biological, inexpensive and rapid process for the fabrication of palladium nanoparticles using the aqueous broth of Pistacia Atlantica fruit as a novel biomass product is reported without using extra surfactant, capping agent, and template. The synthesized palladium nanoparticles were confirmed and characterized by various spectroscopic techniques including UV-Visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectrometer, Fourier transform infrared spectroscopy and Zeta-potential measurement. The results indicate that the spherically shaped Pd nanoparticles were successfully prepared in aqueous media in accordance with the principles of green chemistry with desired stability and crystalline in nature with face centered cubic geometry. Also, the results of transmission electron microscopy (TEM) confirmed preparation of very stable nanoparticles with the small diameter below 15 nm.