A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite


Soldatkina O. V. , Soldatkin O. O. , Kasap B. O. , Kucherenko D. Y. , Kucherenko I. S. , AKATA KURÇ B. , ...More

NANOSCALE RESEARCH LETTERS, vol.12, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12
  • Publication Date: 2017
  • Doi Number: 10.1186/s11671-017-2026-8
  • Title of Journal : NANOSCALE RESEARCH LETTERS
  • Keywords: Silicalite, Enzyme, Biosensor, Glutamate oxidase, Amperometry, FIELD-EFFECT TRANSISTORS, FLOW-INJECTION ANALYSIS, UREA BIOSENSOR, PERSPECTIVE MATERIAL, IMMOBILIZED UREASE, ANALYSIS SYSTEM, SUGAR ANALYSIS, ZEOLITE, ELECTRODES, CREATION

Abstract

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 mu M, and the limit of glutamate detection was 1 mu M. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.