Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection


Gokoglan T. C. , SÖYLEMEZ S., Kesik M., Toksabay S., TOPPARE L. K.

FOOD CHEMISTRY, vol.172, pp.219-224, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 172
  • Publication Date: 2015
  • Doi Number: 10.1016/j.foodchem.2014.09.065
  • Title of Journal : FOOD CHEMISTRY
  • Page Numbers: pp.219-224
  • Keywords: Amperometric biosensor, Conducting polymer based biosensor, Glucose biosensor, Pyranose oxidase, ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY, REGION

Abstract

A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detection of consumed oxygen was monitored at -0.7 V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). K-M(app), I-max, LOD and sensitivity were calculated as 0.229 mM, 42.37 nA, 3.3 x 10 (4) nM and 6.4 nA/mM cm(2), respectively. Scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV) techniques were used to monitor changes in surface morphologies and to run electrochemical characterisations. Finally, the constructed biosensor was applied for the determination of glucose in beverages successfully. (C) 2014 Elsevier Ltd. All rights reserved.