Conjugated polymer (CP)-based biosensors are recognized to be a next generation building architecture for highly sensitive and fast biosensing systems. This entry highlights an overview on the recent expansion of research in the field of CP-based biosensors. We start by first introducing some of the important knowledge about the CPs and biosensors. This is followed by emphasizing the importance of CPs in biosensor construction. Lastly, an overall survey of CP-based biosensor studies reported between 2010 and 2015 is discussed. Several reviews in literature mostly either refer to a specific enzyme or focus on the techniques of biosensor construction together with general information on conducting polymers. Hence, we tend to emphasize on a number of enzymes studied for the past 5 years.