Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity


AKSOY E., UNCU A. T. , FİLİZ E., ORMAN Ş., ÇETİN D., AKBUDAK M. A.

MOLECULAR BIOLOGY REPORTS, vol.49, no.6, pp.5303-5313, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 6
  • Publication Date: 2022
  • Doi Number: 10.1007/s11033-021-06952-3
  • Journal Name: MOLECULAR BIOLOGY REPORTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.5303-5313
  • Keywords: Brachypodium distachyon, Cadmium toxicity, mRNA surveillance, Heavy metal stress, RNAseq, INDUCED OXIDATIVE STRESS, ARABIDOPSIS, GLUTATHIONE, TOLERANCE, CYCLE, HOMEOSTASIS, TRANSPORT, RESPONSES, INSIGHTS, SELENIUM
  • Middle East Technical University Affiliated: Yes

Abstract

Background Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. Methods and results In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins, heavy metal-associated proteins and NRAMP proteins. The Arabidopsis orthologs of the differentially expressed B. distachyon genes (DEGs) under Cd toxicity were identified, which exhibited Bradi4g26905 was an ortholog of AtALY1-2. Detailed co-expression network and gene ontology analyses found the potential involvement of the mRNA surveillance pathway in Cd tolerance in B. distachyon. These genes were shown to be downregulated by sulfur (S) deficiency. Conclusions This is the first transcriptomic study investigating the effect of Cd toxicity in B. distachyon, a model plant for genomic studies in Poaceae (Gramineae) species. The results are expected to provide valuable information for more comprehensive research related to heavy metal toxicity in plants.