Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1

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Papini D., Fant X., Ogawa H., Desban N., Samejima K., Feizbakhsh O., ...Daha Fazla

JOURNAL OF CELL SCIENCE, cilt.132, sa.21, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 132 Sayı: 21
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1242/jcs.234401
  • Dergi Adı: JOURNAL OF CELL SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: CPC, INCENP, Furrow initiation, Aurora B, Plk1, Mitosis, CHROMOSOMAL PASSENGER COMPLEX, CENTROMERE PROTEIN INCENP, POLO-LIKE KINASE-1, AURORA-B, INNER CENTROMERE, CONTRACTILE RING, MITOTIC SPINDLE, RHOGEF ECT2, SPATIOTEMPORAL REGULATION, GENETIC-ANALYSIS
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.