Resorufin Enters the Photodynamic Therapy Arena: A Monoamine Oxidase Activatable Agent for Selective Cytotoxicity

Almammadov T., ATAKAN G., Leylek O., Ozcan G., Gunbas G., Kolemen S.

ACS MEDICINAL CHEMISTRY LETTERS, cilt.11, sa.12, ss.2491-2496, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1021/acsmedchemlett.0c00484
  • Dergi Adı: ACS MEDICINAL CHEMISTRY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE
  • Sayfa Sayıları: ss.2491-2496
  • Anahtar Kelimeler: Photodynamic therapy, activatable photosensitizers, resorufin, cancer cell selectivity, monoamine oxidase, SINGLET OXYGEN, CELL-DEATH, PHOTOSENSITIZER, GENERATION, PROBES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A red-absorbing, water-soluble, and iodinated resorufin derivative (R1) that can be selectively activated with a monoamine oxidase (MAO) enzyme was synthesized, and its potential as a photodynamic therapy (PDT) agent was evaluated. R1 showed high O-1(2) generation yields in aqueous solutions upon addition of MAO isoforms, and it was further tested in cell culture studies. R1 induced photocytotoxicity after being triggered by endogenous MAO enzyme in cancer cells with a much higher efficiency in SH-SYSY neuroblastoma cells with high MAO-A expression. Additionally, R1 displayed differential cytotoxicity between cancer and normal cells, without any considerable dark toxicity. To the best of our knowledge, R1 marks the first example of a resorufin-based photosensitizer (PS) as well as the first anticancer drug that is activated by a MAO enzyme. Remarkably, the target PDT agent was obtained only in three steps as a result of versatile resorufin chemistry.