Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.88, sa.9, ss.1631-1640, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 88 Sayı: 9
- Basım Tarihi: 2013
- Doi Numarası: 10.1002/jctb.4011
- Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1631-1640
- Anahtar Kelimeler: P.pastoris, human growth hormone, sorbitol, methanol, feeding rate, feeding strategy, ALCOHOL OXIDASE, CARBON-SOURCES, PROTEINS, METHANOL, SECRETION, BATCH
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.