Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

ÇALIK, PINAR; Bozkurt, Bahar; Zerze, Gul; Inankur, Bahar; Bayraktar, Eda; BOY, ERDEM; Orman, Mehmet; Acik, Eda; Ozdamar, Tuncer

doi:10.1002/jctb.4011

Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

ÇALIK P. , Bozkurt B., Zerze G. H. , Inankur B., Bayraktar E., BOY E. , ...More

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.88, no.9, pp.1631-1640, 2013 (Journal Indexed in SCI)

Publication Type: Article / Article
Volume: 88 Issue: 9
Publication Date: 2013
Doi Number: 10.1002/jctb.4011
Title of Journal : JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Page Numbers: pp.1631-1640

Abstract

BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.