Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

ÇALIK P., Bozkurt B., Zerze G. H., Inankur B., Bayraktar E., BOY E., ...More

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.88, no.9, pp.1631-1640, 2013 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 88 Issue: 9
  • Publication Date: 2013
  • Doi Number: 10.1002/jctb.4011
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1631-1640
  • Keywords: P.pastoris, human growth hormone, sorbitol, methanol, feeding rate, feeding strategy, ALCOHOL OXIDASE, CARBON-SOURCES, PROTEINS, METHANOL, SECRETION, BATCH
  • Middle East Technical University Affiliated: Yes

Abstract

BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.