Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus alpha-galactosidase

Gurkok S., ÇEKMECELİOĞLU D., Ogel Z. B.

BIORESOURCE TECHNOLOGY, vol.102, no.7, pp.4925-4929, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 102 Issue: 7
  • Publication Date: 2011
  • Doi Number: 10.1016/j.biortech.2011.01.036
  • Journal Name: BIORESOURCE TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.4925-4929
  • Keywords: alpha-Galactosidase, Aspergillus, Optimization, Response surface methodology (RSM), Box-behnken design (BBD), NIGER, GUAR
  • Middle East Technical University Affiliated: Yes

Abstract

Using Response Surface Methodology, carbon and nitrogen sources and agitation speed for cultivation of Aspergillus sojae expressing the alpha-galactosidase gene, aglB of Aspergillus fumigatus IMI 385708 were optimized. Compared to cultivation in modified YpSs medium, cultivation in 250-mL Erlenmeyer flasks agitated at 276 rpm and containing 100 mL of optimized medium consisting of 10.5% molasses (w/v) and 1.3% NH4NO3 (w/v), 0.1% K2HPO4, and 0.005% MgSO4 center dot 7H(2)0 achieved a 4-fold increase in alpha-galactosidase production (10.4 U/mL). These results suggest the feasibility of industrial large scale production of an alpha-galactosidase known to be valuable in galactomannan modification. (C) 2011 Elsevier Ltd. All rights reserved.