Poly(pyrrole) versus poly(3,4-ethylenedioxythiophene): amperometric cholesterol biosensor matrices

Tuerkarslan O., Kayahan S. K., TOPPARE L. K.

JOURNAL OF SOLID STATE ELECTROCHEMISTRY, cilt.13, sa.5, ss.657-663, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 5
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s10008-008-0590-7
  • Dergi Adı: JOURNAL OF SOLID STATE ELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.657-663
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Two conducting polymers, poly(pyrrole) (PPy) and poly(3,4-ethylenedioxythiophene) (PEDOT) were used as immobilization matrices for cholesterol oxidase (ChOx). The amperometric responses of the enzyme electrodes were measured by monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, such as K (m) and I (max), operational and storage stabilities, effects of pH and temperature were determined for both entrapment supports. K (m) values are found as 7.9 and 1.3 mM for PPy and PEDOT enzyme electrodes, respectively; it can be interpreted that ChOx immobilized in PEDOT shows higher affinity towards the substrate.