Methanol feeding strategy design enhances recombinant human growth hormone production by Pichia pastoris

Gunes H., BOY E., Ata O., Zerze G. H., ÇALIK P., Ozdamar T. H.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.91, no.3, pp.664-671, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.1002/jctb.4619
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.664-671
  • Keywords: Biochemical engineering, Bioprocesses, Biotechnology, Bioreactors, Fermentation, FED-BATCH, PROTEIN-PRODUCTION, CARBON-SOURCES, FERMENTATION, EXPRESSION, SORBITOL, GLUCOSE, METABOLISM, MANNITOL, PROMOTER
  • Middle East Technical University Affiliated: Yes

Abstract

BACKGROUND: The effects of designed methanol and co-substrate feeding strategies on fed-batch recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated.