The present study examines the reliability and validity of the Turkish version of the Lifespan Sibling Relationship Scale (LSRS; Riggio, 2000). A total of 578 (336 female, 242 male) Turkish emerging adults participated in this study. A Confirmatory Factor Analysis (CFA) was used to test construct validity for the original six-factor model of the scale with 48 items. Results of the CFA indicated a good model fit. Furthermore, the second-order CFA result showed that the scale can be scored for both the subdimensions and the test as a whole. Multi-group CFA result revealed that the measured construct is invariant across the genders. The results suggested that the Turkish version of the LSRS had adequate internal consistency and construct validity, indicating that it can be reliably used to measure attitudes toward sibling relationship in emerging adulthood among a Turkish population.