Development of a paper-type tyrosinase biosensor for detection of phenolic compounds


Senyurt O., EYİDOĞAN F., Yilmaz R., Oz M. T. , Ozalp V. C. , Arica Y., ...Daha Fazla

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.62, ss.132-136, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 62 Konu: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/bab.1246
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Sayfa Sayıları: ss.132-136

Özet

A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.