Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

Senyurt O., EYİDOĞAN F., Yilmaz R., Oz M. T. , Ozalp V. C. , Arica Y., ...More

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol.62, no.1, pp.132-136, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1002/bab.1246
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.132-136
  • Keywords: 4-chlorophenol, catechol, cresol, l-dopamine, paper strip, phenol, REMOTE ELECTROCHEMICAL BIOSENSOR, MUSHROOM TYROSINASE, FILM, IMMOBILIZATION, CHITOSAN
  • Middle East Technical University Affiliated: Yes


A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.