Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

Senyurt O., EYİDOĞAN F., Yilmaz R., Oz M. T., Ozalp V. C., Arica Y., ...Daha Fazla

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.62, sa.1, ss.132-136, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/bab.1246
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.132-136
  • Anahtar Kelimeler: 4-chlorophenol, catechol, cresol, l-dopamine, paper strip, phenol, REMOTE ELECTROCHEMICAL BIOSENSOR, MUSHROOM TYROSINASE, FILM, IMMOBILIZATION, CHITOSAN
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.