Synthesis and characterization of whitlockite from sea urchin skeleton and investigation of antibacterial activity

Yucel A., Sezer S., BİRHANLI E., Ekinci T., Yalman E., Depci T.

CERAMICS INTERNATIONAL, vol.47, no.1, pp.626-633, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.1016/j.ceramint.2020.08.170
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.626-633
  • Keywords: Sea urchin skeleton, Bioceramic, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, ZINC-OXIDE, ANTIMICROBIAL ACTIVITY, PARTICLE-SIZE, ZNO, SURFACE, MAGNESIUM, HYDROXYAPATITE, TRANSFORMATION, DENSIFICATION, BIOACTIVITY
  • Middle East Technical University Affiliated: No


In the present study, undoped whitlockite and ZnO doped-Whitlockite, which is the second most abundant inorganic material in bone structure, were synthesized from sea urchin skeleton. The obtained bioceramic ma-terials were characterized by XRD, FT-IR, and SEM and their antibacterial activities were determined using the inhibition zone diameters of Escherichia coli and Pseudomonas aeruginosa as gram negative bacteria and Staphylococcus aureus as gram positive bacterium after 24 h incubation. The characterization studies showed that nano size homogenous biocereamic whitlockite (Ca2.86Mg0.14(PO4)(2)) was synthesized from the sea urchin skeleton. After dopping process, the main structure of the whitlockite keeps stable, showing a dopping concentration-independent character. On the other hand, the peaks belonging to ZnO were started to seen in the XRD pattern with increasing the level of ZnO-concentration (after 7 %). All experimental results point out that the obtained whitlockites are viable nominate candidates for bioceramic materials and the results of antibacterial sensitivity prove the inhibitory effect towards Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus for ZnO-doped-whitlockite.