Enhancing the value of nitrogen from rapeseed meal for microbial oil production

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Kiran E. U., Salakkam A., Trzcinski A. P., Bakir U., Webb C.

ENZYME AND MICROBIAL TECHNOLOGY, vol.50, pp.337-342, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 50
  • Publication Date: 2012
  • Doi Number: 10.1016/j.enzmictec.2012.03.004
  • Journal Name: ENZYME AND MICROBIAL TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.337-342
  • Keywords: Rapeseed meal, Fermentation, Solid state fermentation, Free amino nitrogen, Rhodosporidium toruloides, Microbial oil, LINOLENIC ACID PRODUCTION, FED-BATCH CULTURE, ASPERGILLUS-ORYZAE, CUNNINGHAMELLA-ECHINULATA, RHODOTORULA-GLUTINIS, LIPID-ACCUMULATION, ORGANIC NITROGEN, BIOCONVERSION, FERMENTATION, GLYCEROL
  • Middle East Technical University Affiliated: Yes

Abstract

Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g(-1) dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g(-1)), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g(-1)), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g(-1)), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g(-1)).